“You can safely assume that you’ve created God in your own image when it turns out that God hates all the same people you do.” -Anne Lamott
“I sometimes think that God, in creating man, somewhat overestimated his ability”
This thought has been criss crossing my mind as of late. I’m not one to particularly believe in god myself but, if one were to strive to such endeavors how and what would you do? How does one grant divinity? How would i do or explain this without succumbing to blasphemy? Better yet how do I do this without appearing as a believer? ( I mean it honestly doesn’t matter what i say as long as its charged by somewhat satirical inflection does it? )
We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and hence should be useful for planning positional cloning experiments.
But, wait I’m getting ahead of myself, what would would we use? I would suggest the Shroud of Turin. What is that you might ask. The shroud is rectangular, measuring approximately 4.4 × 1.1 m (14.3 × 3.7 ft). The cloth is woven in a three-to-one herringbone twill composed of flax fibrils. Its most distinctive characteristic is the faint, yellowish image of a front and back view of a naked man with his hands folded across his groin. The two views are aligned along the midplane of the body and point in opposite directions. The front and back views of the head nearly meet at the middle of the cloth. i.e. this is apparently Jesus the Nazaranian.
So from this we should be able to gather some sort of DNA sample however small. So, the chromosomal position of a gene targeted for positional cloning is typically defined by the closest flanking crossover events. So, if a gene is to be pinpointed to a defined segment size, a minimum of two crossovers are required, one on either side of the target gene. Theoretically, by having an estimate of the kilobase to centimorgan ratio for a particular genome or genomic region, one can estimate the number of meiotic gametes that one must sample to narrow the position of the gene to a prescribed physical segment of DNA. Surprisingly, despite the large number of genes that have been isolated by positional cloning and the popularity of this technique, we have been unable to find a published formula for making this calculation. It is for this reason we herein describe the derivation of such a formula and its application to positional cloning.
…to be continued